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李曉華,黃粵,馬婷婷,皮婷,姚家成,夏珍珍,馮喆,溫馨.具有抗稻瘟病菌活性的鏈霉菌ⅠT2菌株的分離鑒定及其attB位點的分析[J].中南民族大學學報自然科學版,2019,(2):193-198
具有抗稻瘟病菌活性的鏈霉菌ⅠT2菌株的分離鑒定及其attB位點的分析
Isolating and identifying of Streptomyces sp.ⅠT2 with antifungal activity to Magnaporthe Grisea and analysis of attB site
  
DOI:10.12130/znmdzk.20190208
中文關鍵詞: 稻瘟病菌  鏈霉菌ⅠT2菌株  attB位點  位點特異性重組
英文關鍵詞: Magnaporthe grisea  Streptomyces sp. IT2 strain  attB site  site specific recombination
基金項目:國家自然科學基金資助項目(31070087;湖北省自然科學基金重點項目(2011CDA079);中央高?;究蒲袠I務費專項(CZY19018)
作者單位
李曉華,黃粵,馬婷婷,皮婷,姚家成,夏珍珍,馮喆,溫馨 中南民族大學 生命科學學院 微生物資源與利用湖北省工程技術研究中心,武漢 430074 
摘要點擊次數: 200
全文下載次數: 186
中文摘要:
      以稻瘟病菌NO-1為指示菌,從神農架地區土壤中篩選得到一株微生物,命名為ⅠT2. 通過形態、生理生化研究和16S rDNA基因序列同源性比對,初步鑒定為鏈霉菌屬. 通過不同發酵條件對鏈霉菌ⅠT2菌株發酵液抑菌活性的影響研究,鏈霉菌ⅠT2菌株較適合的發酵條件為:發酵溫度為26.5~30 ℃,發酵轉速為150 r/min,發酵時間為48 h. 利用PCR擴增獲得ⅠT2菌株的attB位點序列,鏈霉菌ⅠT2菌株的attB位點序列長277 bp,它與S. griseus M095 的attB 位點核心區域的序列的相似度最高,達到97%,具有發生交換的核心區域5'-TT. 含有ΦC31attP 位點的整合型質粒pSET152可通過attP位點與鏈霉菌ⅠT2菌株的染色體上的attB位點發生位點特異性重組.
英文摘要:
      A strain of microorganism was screened from the soil of Shennongjia area with Magnaporthe grisea NO-1 as indicator, which was named as IT2. It was identified as Streptomyces by morphological, physiological, biochemical studies and 16S rDNA gene sequence. The effect of different culture conditions on the antibacterial activity of the IT2 strain fermentation broth was determined by cup and saucer method. The results showed that the fermentation conditions of Streptomyces sp. IT2 strain were as follows: the fermentation temperature was 26.5-30 °C, the fermentation speed was 150 r/min, and the fermentation time was 48 h. The attB locus sequence of the IT2 strain was obtained by PCR amplification. The attB locus sequence of the Streptomyces sp. IT2 strain was 277 bp in length, which had the highest similarity to the sequence of the attB locus core region of S. griseus M095, reaching 97%. The integrative plasmid pSET152 including ΦC31 attP site recombined specifically between the attP site in pSET152 and the attB site in the chromosome of Streptomyces sp. IT2 strain.
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