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脊髓灰質炎病毒I型抗體膠體金檢測試紙條的研制
Development of poliovirus type I antibody colloidal gold test strip
投稿時間:2019-05-05  修訂日期:2019-05-05
DOI:
中文關鍵詞: 脊髓灰質炎病毒  單克隆抗體  膠體金試紙條
英文關鍵詞: poliovirus  monoclonal antibody  colloidal gold test strip
基金項目:中央高?;究蒲袠I務費專項資金資助項目(37001/CZP17010);中南民族大學橫向科研資助項目(HZY17059)
作者單位E-mail
王朝元 中南民族大學生命科學院 wangchaoyuan@mail.scuec.edu.cn 
黃圣 中南民族大學生命科學院  
尹新濤 武漢生命科技股份有限公司  
項雅利 武漢生命科技股份有限公司  
尹玉瑩 中南民族大學生命科學院  
莫嬌 中南民族大學生命科學院  
摘要點擊次數: 946
全文下載次數: 0
中文摘要:
      通過膠體金免疫層析技術建立一種特異、快速的脊髓灰質炎病毒抗體檢測方法,對接種脊灰疫苗后的抗體水平監測提供參考.用辛酸-硫酸銨沉淀法純化脊灰單抗,用檸檬酸三鈉還原法制備的膠體金,標記純化單抗,再結合脊灰抗原形成復合物,固定于金標墊上,將鼠抗人IgG和羊抗鼠IgG分別包被于試紙條的 T (檢測線) 處和 C (質控線) 處,組裝試紙條.應用該試紙條進行臨床樣品檢測.結果表明,以稀釋后的脊灰陽性血清作為檢測樣品,試紙條檢測的靈敏度為1:80;特異性試驗證明該試紙條與甲型肝炎病毒、乙型肝炎病毒、麻疹病毒抗體陽性血清沒有交叉反應;在4℃和室溫條件下保存3個月,試紙條的靈敏度和特異性沒有明顯改變,說明試紙條穩定性良好;檢測結果與脊灰疫苗抗體檢測試劑盒(ELISA)試驗的符合率為86.7%.表明該脊灰病毒抗體膠體金檢測試紙可以方便、快速、靈敏、特異性地檢測脊灰病毒的抗體水平,適用于臨床大規模血清抗體水平監測,具有良好的應用前景.
英文摘要:
      A specific and rapid detection method of poliovirus antibody was established by colloidal gold immunochromatography, which provides a reference for monitoring antibody levels after polio vaccination. Polio monoclonal antibody was purified with caprylic acid-ammonium sulfate precipitation method and colloidal gold was prepared by trisodium citrate reduction method. Colloidal gold labeled purified monoclonal antibody was combined with polio antigen to form complex, which immobilized on gold standard pad. Mouse anti-human IgG and the goat anti-mouse IgG was coated on the T (test line) and C (quality control line) of the test strip respectively, and the test strip was assembled. The test strip was used for sample test and the results showed that the sensitivity of the test strip was 1:800 after the diluted polio-positive serum was used as the test sample; the specificity test results proved that the test strip did not cross-react and the hepatitis A virus, Hepatitis B virus and measles virus antibody positive serum; after stored for 3 months at 4 ℃ and room temperature, the sensitivity and specificity of the test strip did not change significantly, indicating that the test strip was stable; The coincidence rate of our results with polio-vaccine antibody detection kit (ELISA) test was 86.7%, which confirmed that the established poliovirus antibody colloidal gold test strip is a conveniently, rapidly, sensitively and specifically method to detect the antibody level of poliovirus. It is suitable for clinical large-scale serum antibody level monitoring, and has good application prospect.
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